Name Course Instructor Date Identifying and isolating microbes from the environment Abstract Microbes are found in different locations and surface types, and researchers have in the past relied on culturing microbes to isolate and identify the microbes. Increasingly, DNA sequencing has been utilized as a more effective way to identify microbial characteristics in the built environment different samples were collected in the biology lab and the hallway where gram staining and 16S rRNA sequencing was conducted to identify the various microbes on the surfaces. After analysis, Micrococcus luteus strain, uncultured bacterium clone and staphylococcus sp strains were identified, but the other five could not be identified. Sequential analysis was possible through the use of FINCH and BLAST programs, and gel image was also utilized for analysis. Human contact was identified as a strong predictor of microbes form the built environment, while spatial proximity, the location and the time the study was conducted could also explain microbial diversity. Introduction Microorganisms are everywhere in the built environment and culturing the microbes is necessary to identify the microbes present in this environment. Even as this approach has been used over time there is a drawback since some microbes cannot be cultured. DNA sequencing is equally cheap and effective since it is possible to identify the microbes when here are no cultures. As human have created the built environm...
