2017 BIO130 Lab Report 04 Worksheet

1.   Use a search engine (eg. Google) on the internet to find information about Anna Anderson, then answer the questions below.  (Total of 30 points)

Who did Anna Anderson claim to be?

Who was Anna Anderson identified to be in a 1927 investigation commissioned by Ernst Louis, the Grand Duke of Hesse and brother of Tsarina Alexandria?  (Hint:  see Wikipedia entry on Anna Anderson) 

What DNA fingerprinting method was used to match Anna Anderson’s DNA to the person above?  (Hint: see Wikipedia entry on Anna Anderson)

Name four applications besides criminal investigations that DNA fingerprinting is used for today.  (40 points)

If you started PCR with a single double-stranded (ds) DNA, how many ds-DNAs would you have after four rounds of PCR amplification?  (15 points). 

How many human STR sites have the FBI designated for use in the “CODIS Standard for DNA Testing”?   (5 points)

In a DNA analysis report, you might see something like this:  “TPOX  4, 5”.  Why are

two numbers for the TPOX locus rather than one number or three numbers?  (10 points).   (Hint:  think about how the nuclear human genome is constructed).